The active site of AChE comprises 2 subsites-the anionic site and the esteratic subsite.
2.
Pralidoxime is able to attach to the other half ( the unblocked, anionic site ) of the acetylcholinesterase enzyme.
3.
The cationic substrates are not bound by a negatively charged amino acid in the anionic site, but by interaction of 14 aromatic residues that line the gorge leading to the active site.
4.
It is generally believed ( though not proven ) that a dendrotoxin molecules bind to anionic sites near the extracellular surface of the channel and physically occlude the pore, thereby preventing ion conductance.
5.
It is believed that these positively charged residues can play a critical role in dendrotoxin binding activity, as they can make potential interactions with the anionic sites ( negatively charged amino acids ) in the pore of potassium channels.
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